期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 73, 期 6, 页码 708-717出版社
OXFORD UNIV PRESS
DOI: 10.1111/lam.13558
关键词
carbapenemase; Escherichia coli; IMI; integrons; rabbit farm; VIM
资金
- Institution of Agricultural Research and Higher Education (IRESA), Tunisia
The study focused on antibiotic susceptibility and the presence of ESBL/carbapenemase-producing E. coli isolates from rabbits in Tunisia. It was found that rabbits may serve as a reservoir for carbapenem-resistant E. coli, and the first report of bla(VIM) and bla(IMP) genes in livestock in Tunisia was identified.
We aimed to study the antibiotic susceptibility and possible occurrence of extended-spectrum beta-lactamases (ESBL)/carbapenemase-producing Escherichia coli isolates collected from rabbits in Tunisia. In all, 35 faecal samples from healthy rabbits were collected from one farm and E. coli were isolated from three media: antibiotic-free TBX agar, TBX+2 mg l(-1) cefotaxime and TBX+1 mg l(-1) imipenem. In total, 39 E. coli isolates were recovered; the majority showed resistance to at least one antibiotic and none was ESBL producer. Carbapenem resistance was detected in 16 isolates from either selective or un-selective media. Phenotypic methods used to detect carbapenemase production showed two positive isolates by Modified Hodge Test, six metallo-carbapenemase producers (Imipenem disc+EDTA) and all were temocillin resistant (possible OXA-48 carbapenemase). bla(VIM) and bla(IMP) type genes were detected in two and one isolates, respectively; one of them harboured both genes. Isolates contained common genes encoding resistance to sulphonamides (sul1, sul2), tetracycline (tetA, tetB, tetC) and fluoroquinolones (qnrS, aac(6 ')-Ib-cr). Class 1 and 2 integrons were detected in five and four isolates, respectively. These findings highlight the importance of rabbit production as reservoir of carbapenem-resistant E. coli and argument the first report of bla(VIM) and bla(IMP) genes in livestock in Tunisia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据