A Study of the Effects of Plasma Surface Treatment on Lipid Bilayers Self-Spreading on a Polydimethylsiloxane Substrate under Different Treatment Times

Plasma-treated poly(dimethylsiloxane) (PDMS)-supported lipid bilayers are used as functional tools for studying cell membrane properties and as platforms for biotechnology applications. Self-spreading is a versatile method for forming lipid bilayers. However, few studies have focused on the effect of plasma treatment on self-spreading lipid bilayer formation. In this paper, we performed lipid bilayer self-spreading on a PDMS surface with different treatment times. Surface characterization of PDMS treated with different treatment times is evaluated by AFM and SEM, and the effects of plasma treatment of the PDMS surface on lipid bilayer self-spreading behavior is investigated by confocal microscopy. The front-edge velocity of lipid bilayers increases with the plasma treatment time. By theoretical analyses with the extended-DLVO modeling, we find that the most likely cause of the velocity change is the hydration repulsion energy between the PDMS surface and lipid bilayers. Moreover, the growth behavior of membrane lobes on the underlying self-spreading lipid bilayer was affected by topography changes in the PDMS surface resulting from plasma treatment. Our findings suggest that the growth of self-spreading lipid bilayers can be controlled by changing the plasma treatment time.

Automated summary

This study investigated the effect of plasma treatment on self-spreading lipid bilayer formation on PDMS surfaces with different treatment times. The results showed that increasing plasma treatment time accelerates the front-edge velocity of lipid bilayers, and changes in the topography of the PDMS surface due to plasma treatment affect the growth of membrane lobes on the underlying bilayer. It suggests that the growth of self-spreading lipid bilayers can be controlled by adjusting the plasma treatment time.