期刊
KIDNEY INTERNATIONAL
卷 101, 期 1, 页码 131-136出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2021.09.004
关键词
biopsy; kidney transplant; spatial transcriptomic
资金
- Renal Research Institute, New York, NY
- National Institute of Allergy and Infectious Diseases [R01 AI141710]
The application of spatial transcriptomics in kidney transplant biopsies allows for the study of variability between different regions in acute cellular rejection, revealing new pathogenic mechanisms related to the graft response to alloimmune attack and providing new therapeutic targets for acute cellular rejection and other kidney diseases with a focal nature.
Analysis of the transcriptional profile of graft biopsies represents a promising strategy to study T cell-mediated-rejection (TCMR), also known as acute cellular rejection. However, bulk RNA sequencing of graft biopsies may not capture the focal nature of acute rejection. Herein, we used the whole exome GeoMX Digital Space Profiling platform to study five tubular and three glomerular regions of interest in the kidney graft biopsy from a patient with a chronic-active TCMR episode and in analogous areas from two different normal kidney control biopsies. All kidney sections were from paraffin blocks. Overall, inflammatory genes were significantly upregulated in the tubular areas of the TCMR biopsy and showed an enrichment for geneontology terms associated with T-cell activation, differentiation, and proliferation. Enrichment analysis of the 100 genes with the highest coefficient of variation across the TCMR tubular regions of interest revealed that these highly variable genes are involved in kidney development and injury and interestingly do not associate with the 2019 Banff classification pathology scores within the individual regions of interest. Spatial transcriptomics allowed us to unravel a previously unappreciated variability across different areas of the TCMR biopsy related to the graft response to the alloimmune attack, rather than to the immune cells. Thus, our approach has the potential to decipher clinically relevant, new pathogenic mechanisms, and therapeutic targets in acute cellular rejection and other kidney diseases with a focal nature.
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