期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 22, 期 7, 页码 575-589出版社
ZHEJIANG UNIV PRESS
DOI: 10.1631/jzus.B2000520
关键词
Black tea; Angiotensin-1-converting enzyme (ACE) inhibitory peptide; Kinetic study; Molecular docking; Molecular dynamic (MD) simulation
类别
资金
- National Key Research and Development Program of China [2016YFD0200900]
- Science Technology Department of Zhejiang Province [2016C02053-8]
The study aims to investigate the inhibitory mechanism of tea peptides on the angiotensin-converting enzyme (ACE) using in vitro and in silico methods. Results showed that four tea peptides act as allosteric inhibitors to ACE, with peptide IV demonstrating the lowest IC50 value possibly due to its stability during docking with ACE. These bioactive peptides have the potential to be used as novel functional ingredients in black tea.
The aim of this work is to discover the inhibitory mechanism of tea peptides and to analyse the affinities between the peptides and the angiotensin-converting enzyme (ACE) as well as the stability of the complexes using in vitro and in silico methods. Four peptide sequences identified from tea, namely peptides I, II, III, and IV, were used to examine ACE inhibition and kinetics. The half maximal inhibitory concentration (IC50) values of the four peptides were (210.03 +/- 18.29), (178.91 +/- 5.18), (196.31 +/- 2.87), and (121.11 +/- 3.38) mu mol/L, respectively. The results of Lineweaver-Burk plots showed that peptides I, II, and IV inhibited ACE activity in an uncompetitive manner, which requires the presence of substrate. Peptide III inhibited ACE in a non-competitive manner, for which the presence of substrate is not necessary. The docking simulations showed that the four peptides did not bind to the active sites of ACE, indicating that the four peptides are allosteric inhibitors. The binding free energies calculated from molecular dynamic (MD) simulation were -72.47, -42.20, -52.10, and -67.14 kcal/mol (1 kcal=4.186 kJ), respectively. The lower IC50 value of peptide IV may be attributed to its stability when docking with ACE and changes in the flexibility and unfolding of ACE. These four bioactive peptides with ACE inhibitory ability can be incorporated into novel functional ingredients of black tea.
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