4.6 Article

Immunophenotyping and Transcriptional Profiling of Human Plasmablasts in Dengue

期刊

JOURNAL OF VIROLOGY
卷 95, 期 23, 页码 -

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AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00610-21

关键词

plasmablasts; dengue; human; RNA-seq; immunophenotyping; India

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资金

  1. U.S. National Institutes of Health [ICIDR 1UO1A/115654]
  2. NIH-DBT, Human Immunology Project Consortium (HIPC) [BT/PR30260/MED/15/194/2018]
  3. Government of India Department of Biotechnology [DBT BT/PR5132/MED/15/85/2012]

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Plasmablasts play a vital role in systemic infections like dengue, regulating the expression of various factors involved in cell interactions, tissue homing, leukocyte extravasation, angiogenesis, and B-cell survival. Their comprehensive transcriptional profiles in dengue patients provide valuable insights into the acute febrile phase of the infection.
Plasmablasts represent a specialized class of antibody-secreting effector B cells that transiently appear in blood circulation following infection or vaccination. The expansion of these cells generally tends to be massive in patients with systemic infections such as dengue or Ebola that cause hemorrhagic fever. To gain a detailed understanding of human plasmablast responses beyond antibody expression, here, we performed immunophenotyping and RNA sequencing (RNA-seq) analysis of the plasmablasts from dengue febrile children in India. We found that plasmablasts expressed several adhesion molecules and chemokines or chemokine receptors that are involved in endothelial interactions or homing to inflamed tissues, including skin, mucosa, and intestine, and upregulated the expression of several cytokine genes that are involved in leukocyte extravasation and angiogenesis. These plasmablasts also upregulated the expression of receptors for several B-cell prosurvival cytokines that are known to be induced robustly in systemic viral infections such as dengue, some of which generally tend to be relatively higher in patients manifesting hemorrhage and/or shock than in patients with mild febrile infection. These findings improve our understanding of human plasmablast responses during the acute febrile phase of systemic dengue infection. IMPORTANCE Dengue is globally spreading, with over 100 million clinical cases annually, with symptoms ranging from mild self-limiting febrile illness to more severe and sometimes life-threatening dengue hemorrhagic fever or shock, especially among children. The pathophysiology of dengue is complex and remains poorly understood despite many advances indicating a key role for antibody-dependent enhancement of infection. While serum antibodies have been extensively studied, the characteristics of the early cellular factories responsible for antibody production, i.e., plasmablasts, are only beginning to emerge. This study provides a comprehensive understanding of the transcriptional profiles of human plasmablasts from dengue patients.

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