期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 92, 期 -, 页码 78-89出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2016.01.010
关键词
Blood-brain barrier; Endothelial cell; Estrogen receptor; Ischemic reperfusion injury; Paracellular permeability; Tight junction
资金
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A6A3A01009345]
- Ministry of Science, ICT & Future Planning [2010-0027945]
- Ewha Womans University
- National Research Foundation of Korea [2010-0027945] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ischemic stroke, which induces oxidative stress in the brain, disrupts tight junctions (TJs) between brain endothelial cells, resulting in blood-brain barrier (BBB) breakdown and brain edema. Estrogen reduces oxidative stress and protects brain endothelial cells from ischemic insult. The aim of this study was to determine the protective effects of estrogen on TJ disruption and to examine the roles of classical estrogen receptor (ER) subtypes, ER alpha- and ER beta, in estrogen effects in brain endothelial cells (bEnd.3) exposed to oxygen-glucose deprivation/reperfusion (OGD/R) injury. Estrogen pretreatment prevented OGD/R-induced decreases in cell viability and TJ protein levels. ER alpha- and ER beta-specific agonists also reduced TJ disruption. Knockdown of ER alpha or ER beta expression partially inhibited the effects of estrogen, but completely reversed the effects of corresponding ER subtype-specific agonists on the outcomes of OGD/R. During the early reperfusion period, activation of extracellular signal-regulated kinase1/2 and hypoxia-inducible factor 1 alpha/vascular endothelial growth factor was associated with decreased expression of occludin and claudin-5, respectively, and these changes in TJ protein levels were differentially regulated by ER subtype-specific agonists. Our results suggest that ERA and EV activation reduce TJ disruption via inhibition of signaling molecules after ischemic injury and that targeting each ER subtype can be a useful strategy for protecting the BBB from ischemic stroke in postmenopausal women. (C) 2016 Elsevier Inc. All rights reserved.
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