Molecularly Imprinted Scaffold Based on poly (3-aminobenzoic acid) for Electrochemical Sensing of Vitamin B6

Inadequate or excess consumption of Vitamin B-6 (Vit B-6) can have ill effects on the overall well-being of humans, thereby making it necessary to control their content and composition in the food we consume. A simple sensor is fabricated in this work for Vit B-6 detection based on employing an electropolymerized molecularly imprinted polymer (MIP) of 3-amino benzoic acid. The poly (3- aminobenzoic acid) (P-(3ABA)) film was electrodeposited by potentiodynamic cycling of potential with and without Vit B-6 (template) on carbon fiber paper electrode (CFP). The modified working electrodes were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Field emission scanning microscopy, Energy dispersive X-ray spectrometry, Fourier Transform Infrared spectroscopy, Optical profilometry and X-ray photon electron spectroscopy were used for characterization. Nyquist plots revealed least charge transfer resistance at MIP/CFP than other control electrodes due to the molecularly imprinted sites. Under the optimized experimental conditions the developed MIP sensor showed a linear range 0.6 mu M to 700 mu M, with a detection limit of 0.010 mu M. Also a value of the imprinting factor (beta = 3.50) indicates very good selectivity of the prepared sensor towards Vit B6 detection over its structurally similar analogues in the analysis of real sample matrices.

Automated summary

A simple sensor for detecting Vitamin B-6 was developed in this study using an electropolymerized molecularly imprinted polymer. The sensor showed good performance and selectivity towards Vitamin B-6 detection based on the characterization of working electrodes.