Microwave-Assisted Automated Glycan Assembly

Automated synthesis of DNA, RNA, and peptides provides quickly and reliably important tools for biomedical research. Automated glycan assembly (AGA) is significantly more challenging, as highly branched carbohydrates require strict regio-and stereocontrol during synthesis. A new AGA synthesizer enables rapid temperature adjustment from -40 to +100 degrees C to control glycosylations at low temperature and accelerates capping, protecting group removal, and glycan modifications using elevated temperatures. Thereby, the temporary protecting group portfolio is extended from two to four orthogonal groups that give rise to oligosaccharides with up to four branches. In addition, sulfated glycans and unprotected glycans can be prepared. The new design reduces the typical coupling cycles from 100 to 60 min while expanding the range of accessible glycans. The instrument drastically shortens and generalizes the synthesis of carbohydrates for use in biomedical and material science.

Automated summary

The new AGA synthesizer offers better temperature control, more protecting group options, and shorter coupling cycles, enabling rapid and efficient synthesis of complex carbohydrates and expanding the range of glycans that can be prepared.