期刊
JOURNAL OF SEPARATION SCIENCE
卷 44, 期 16, 页码 3061-3069出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100092
关键词
anticoagulant component; carthami flos; high performance liquid chromatography; hydroxysafflor yellow A; thrombin affinity chromatography
资金
- China Postdoctoral Science Foundation [2018M643681]
- National Natural Science Foundation of Shaanxi Province [2017JQ8024, 2019SF-153]
- Shaanxi Province Innovative Talent Promotion Plan [2019TD-017]
The study revealed that hydroxysafflor yellow A, an active component in safflower, has anticoagulant effects and can prolong clotting time by interacting with thrombin, making it a potential anticoagulant drug for the treatment of thrombosis.
Carthami flos, commonly known as Honghua in China, is the dried floret of safflower and widely acknowledged as a blood stasis promoting herb. The study aimed at investigating the relationship between thrombin and carthami flos through a high-performance thrombin affinity chromatography combined with a high-performance liquid chromatography-tandem mass spectrometry system. First, thrombin was immobilized on the glutaraldehyde-modified amino silica gel to prepare the thrombin affinity stationary phase, which was packed into a small column (1.0 x 2.0 mm, id) for recognizing the anticoagulant active components of carthami flos. The target component was enriched and analyzed by the high-performance liquid chromatography-tandem mass spectrometry system. Finally, hydroxysafflor yellow A was screened out and identified as the active component. The anticoagulant effects of hydroxysafflor yellow A were analyzed by anticoagulant experiments in vitro, and the interaction of hydroxysafflor yellow A with thrombin was investigated by the molecular docking method. The results proved that hydroxysafflor yellow A (30 mu g/mL, 0.05 mM) and carthami flos extract (30 mu g/mL) could prolong activated partial thrombin time and thrombin time by 50 and 11%, respectively. Moreover, hydroxysafflor yellow A exhibits a good hydrogen bond field and stereo field matching with thrombin. Overall, it was concluded that hydroxysafflor yellow A might exert an anticoagulation effect by interacting with thrombin and thus could be potential anticoagulant drugs for the prevention and treatment of venous thrombosis.
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