期刊
JOURNAL OF SEPARATION SCIENCE
卷 44, 期 17, 页码 3237-3247出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100286
关键词
diastereoisomers; Eupatorium lindleyanum; pyrrolizidine alkaloids; risk assessment
资金
- 13th 5-Year National Significant New Drugs Creation Feature Subjects [2018ZX09735006]
- National Institutes for Food and Drug Control [2020A3]
A new ultra high performance liquid chromatography tandem mass spectrometry technique was developed to detect eight pyrrolizidine alkaloids in Eupatorium lindleyanum, with a risk assessment method also explored. Variations were found in the levels of these alkaloids in different parts of the plant, with the highest levels in the roots followed by flowers, stems, and leaves. The risk assessment data suggested minimal harmful effects on human health with shorter exposure times during the plant's lifetime.
Pyrrolizidine alkaloids are toxins having hepatotoxic and carcinogenic effects on human health. A ultra high performance liquid chromatography tandem mass spectrometry technique was developed for the first time for the simultaneous determination of eight pyrrolizidine alkaloids, including four diastereoisomers (intermedine, lycopsamine, rinderine, and echinatine) and their respective N-oxide forms, in different parts of Eupatorium lindleyanum. The risk assessment method for pyrrolizidine alkaloids in Eupatorium lindleyanum was explored using the margin of exposure strategy for the first time based on a real-life exposure scenario. Differences were found in all eight pyrrolizidine alkaloids in various parts of Eupatorium lindleyanum. Besides, the total levels of pyrrolizidine alkaloids in Eupatorium lindleyanum followed the order of root > flower > stem > leaf. Moreover, the risk assessment data revealed that the deleterious effects on human health were unlikely at exposure times of less than 200, 37, and 12 days during the lifetimes of Eupatorium lindleyanum leaves, stems, and flowers, respectively. This study reported both the contents of and risk associated with Eupatorium lindleyanum pyrrolizidine alkaloids. The comprehensive application of the novel ultra high performance liquid chromatography tandem mass spectrometry technique alongside the risk assessment approach provided a scientific basis for quality evaluation and rational utilization of toxic pyrrolizidine alkaloids in Eupatorium lindleyanum to improve public health safety.
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