期刊
JOURNAL OF SEPARATION SCIENCE
卷 44, 期 15, 页码 2888-2897出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100291
关键词
double chain amide phase; mixed-mode stationary phase; reversed-phase; hydrophilic interaction mode; single-chain amide phase
Conventional reactions were used to functionalize the silica surface, resulting in two different mixed-mode stationary phases which showed different interactions under distinct chromatographic conditions. The retention of various analytes was investigated, leading to efficient separation based on the type of amide group and chromatography mode used.
In this work, the conventional reactions were used to functionalize the silica surface with amide and hydrocarbon chain groups affording two different mixed-mode stationary phases (Sil-amide-C11 and Sil-C12-amide). The prepared stationary phases were analyzed by elemental analysis and thermogravimetric analysis. The retention of benzene, phenol, pyridine, and aniline was investigated and compared with synthesized and commercial columns, and this led to prove the existence of different interactions on the synthesized stationary phases. The mixed-mode stationary phases showed multiple interactions, and different chromatography modes were found under distinct chromatographic conditions. According to the type of amide group (either free or within the hydrocarbon chain), different interactions can be made on the columns. The alkylbenzenes and polycyclic aromatic hydrocarbons, as nonpolar hydrocarbons, were chromatographed under reversed-phase liquid chromatography modes, in which amide groups on the silica could efficiently separate polar analytes under hydrophilic interaction liquid chromatography mode in both prepared stationary phases. The performance of the columns was compared by the separation of the carboxylic acid group and biological samples. The bonding method and the type of amide group showed different interactions leading to different separation and performance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据