Purification and radioiodination of 2, 4 di-tertiary- butyl phenol extracted from Lactococcus lactis subsp. lactis CAU: 3138-GM2 and its application on myeloma cells

The goal of the present study was oriented to extraction of a bioactive compound 2,4-di-tertiary-butyl phenol (2,4-DTBP) from Lactococcus lactis subsp. lactis CAU: 3138-GM2 of goat milk origin (accession no. MF354327.1). The 2,4-DTBP was extracted by methanol: chloroform. Radioiodination of 2,4-DTBP using (NaI)-I-125 and chloramine -T was carried out. Effect of radiolabeled tracer and non-radiolabeled of 2,4-DTBP on the inhibition of growth myeloma cells were evaluated. The bioactivity of 2,4-DTBP was tested against Staphylococcus aureus MRSA ATCC 43300, Listeria monocytogenes ATCC 19116, Pseudomonas aeruginosa MTCC 1934, and Escherichia coli MTCC 1610, Clostridium bifermentans MTCC 11273 and Candida albicans MTCC 183.

Automated summary

The present study aimed to extract a bioactive compound 2,4-di-tertiary-butyl phenol (2,4-DTBP) from goat milk origin and radioiodinate it. The inhibitory effect of 2,4-DTBP on myeloma cell growth was evaluated, and its antimicrobial activity was tested against various bacteria and fungi.