Proteomic analysis of individual giant freshwater prawn, Macrobrachium rosenbergii, growth retardants

Iron prawn is a condition of severe growth retardation that fishers call. The giant river prawn (Macrobrachium rosenbergii) is a commercially important species contains high protein content and functional nutrients. However, no proteomic information is available for this species. We performed the shotgun 2DLC-MS/MS proteomic analysis of the total protein from iron prawn. Total 19,758 peptides corresponding to 2613 high-confidence proteins were identified. These proteins range in size from 40 to 70 kDa. KEGG analysis revealed that the largest group consisting total 102 KEGG pathway proteins comparing the iron prawn with the normal prawn. Additionally, 7, 11, 1, 6, and 5 commercially important enzymes were found in the eyestalk, liver, muscle, ovary, and testis, respectively. The functions of these differently expressed enzymes include immune system action against pathogens, muscle contraction, digestive system metabolism, cell differentiation, migration, and apoptosis in the severe growth retardation of iron prawn. Our work provides insight into the understanding of the formation mechanism of iron prawn.

Automated summary

This study conducted proteomic analysis on iron prawn and identified a large number of differently expressed proteins, which play various functional roles in the severe growth retardation of iron prawn.