4.7 Review

Peptidomics: A Review of Clinical Applications and Methodologies

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Summary: LC-MS/MS peptidomic analysis of murine EECs and human GI tissue identified a variety of peptides, including known gut hormones and enzymatic degradation products. EEC-specific peptides were mainly derived from vesicular proteins, particularly prohormones, granins, and processing enzymes, with some showing regulated secretion in vitro. High fat feeding impacted peptide levels from GCG, PYY, and INSL5, particularly in the distal colon. Two candidate novel peptides tested in vivo had minor effects, with a progastrin-derived peptide modestly improving glucose tolerance in lean mice.

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NATURE COMMUNICATIONS (2016)

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Plasma Proteome Profiling to Assess Human Health and Disease

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CELL SYSTEMS (2016)

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Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling

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ANALYTICAL CHEMISTRY (2015)

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LC-MS/MS method for hepcidin-25 measurement in human and mouse serum: clinical and research implications in iron disorders

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CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2015)

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Stability of glucagon-like peptide 1 and glucagon in human plasma

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ENDOCRINE CONNECTIONS (2015)

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Comprehensive Quantitative Analysis of Ovarian and Breast Cancer Tumor Peptidomes

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Optimization for peptide sample preparation for urine peptidomics

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BIOINFORMATICS (2013)

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Extensive in vivo Human Milk Peptidomics Reveals Specific Proteolysis Yielding Protective Antimicrobial Peptides

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JOURNAL OF PROTEOME RESEARCH (2013)

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Potentials and pitfalls of clinical peptidomics and metabolomics

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SWISS MEDICAL WEEKLY (2013)

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Peptidomics The Comprehensive Analysis of Peptides in Complex Biological Mixtures

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LC-MS/MS for protein and peptide quantification in clinical chemistry

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Evaluation of Database Search Programs for Accurate Detection of Neuropeptides in Tandem Mass Spectrometry Experiments

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PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification

Jing Zhang et al.

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Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides

Yun-Gon Kim et al.

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Review Biotechnology & Applied Microbiology

Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices

Inez Finoulst et al.

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2011)

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A Face in the Crowd: Recognizing Peptides Through Database Search

Jimmy K. Eng et al.

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Peptidomics of the Prolyl Peptidases

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AAPS JOURNAL (2010)

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Differences in metabolite profile between blood plasma and serum

Linsheng Liu et al.

ANALYTICAL BIOCHEMISTRY (2010)

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Peptidomics Coming of Age: A Review of Contributions from a Bioinformatics Angle

Gerben Menschaert et al.

JOURNAL OF PROTEOME RESEARCH (2010)

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Strategies to prolong the plasma residence time of peptide drugs

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MEDCHEMCOMM (2010)

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Study of the human plasma proteome of rheumatoid arthritis

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A label-free nano-liquid chromatography-mass spectrometry approach for quantitative serum peptidomics in Crohn's disease patients

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Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics

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Urinary Peptidome May Predict Renal Function Decline in Type 1 Diabetes and Microalbuminuria

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Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LC-MS/MS

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Hypo-Phosphorylation of Salivary Peptidome as a Clue to the Molecular Pathogenesis of Autism Spectrum Disorders

Massimo Castagnola et al.

JOURNAL OF PROTEOME RESEARCH (2008)

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Enrichment of low molecular weight serum proteins using acetonitrile precipitation for mass spectrometry based proteomic analysis

Richard Kay et al.

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Observation of peptide differences between cancer and control in gastric juice

Wei-Chao Chang et al.

PROTEOMICS CLINICAL APPLICATIONS (2008)

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Serum and plasma proteomics

Haleem J. Issaq et al.

CHEMICAL REVIEWS (2007)

Article Biochemical Research Methods

Improvement of recovery and repeatability in liquid chromatography-mass spectrometry analysis of peptides

Paul M. van Midwoud et al.

JOURNAL OF PROTEOME RESEARCH (2007)

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Evaluation of endogenous plasma peptide extraction methods for mass spectrometric biomarker discovery

Lina P. Aristoteli et al.

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SwePep, a database designed for endogenous peptides and mass spectrometry

Maria Falth et al.

MOLECULAR & CELLULAR PROTEOMICS (2006)

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Validation of breast cancer biomarkers identified by mass spectrometry

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Identification of novel biomarker candidates by differential peptidomics analysis of cerebrospinal fluid in Alzheimer's disease

H Selle et al.

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Quantitation of the large polypeptide glucagon by protein precipitation and LC/MS

DC Delinsky et al.

BIOMEDICAL CHROMATOGRAPHY (2004)

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Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry

C Polson et al.

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Effect of anticoagulants and storage temperatures on stability of plasma and serum hormones

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