期刊
JOURNAL OF PLANT PATHOLOGY
卷 103, 期 4, 页码 1315-1321出版社
SPRINGER
DOI: 10.1007/s42161-021-00906-1
关键词
16S-23S ITS region; Leucyl tRNA synthetase gene; Rapid detection
A new set of loop-mediated isothermal amplification assays has been developed and optimized for rapid laboratory detection of phytoplasmas associated with plant diseases, targeting specific phytoplasma 16Sr groups. These assays are specific, sensitive, and capable of providing results within 30 minutes in real-time LAMP assays.
A new set of loop-mediated isothermal amplification assays was developed for detection of phytoplasmas associated with plant diseases and optimised for rapid laboratory detection. This set of assays was designed based on the leucyl tRNA synthetase gene expressed in most of the phytoplasmas and showing a good level of sequence divergence among strains from different 16Sr groups. This makes it a good target for developing discrete assays that can detect specific phytoplasma 16Sr groups. In this study, unique sets of LAMP assays were designed and validated for seven phytoplasma ribosomal groups (16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, 16SrX, and 16SrXII). These assays are specific, rapid and as sensitive as the nested PCR assays and the results could be obtained in less than 30 min in real-time LAMP assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据