Vanillic acid Zn(II) complex: a novel complex with antihyperglycaemic and anti-oxidative activity

Objectives Our aim was to synthesize, characterize and evaluate the antihyperglycaemic and antioxidative properties of a new Zn(II) complex of vanillic acid. Methods The complex was synthesized using ZnSO4 center dot 7H(2)O and vanillic acid as precursors. NMR and FTIR techniques were used to characterize the synthesized complex. The cytotoxicity of the complex was measured.The antihyperglycemic and anti-oxidative properties of the complex were evaluated using in vitro, cell-based and ex vivo models and compared with those of its precursors. Key findings Zn(II) coordinated with vanillic acid via a Zn(O-6) coordination, with the complex having three moieties of vanillic acid. The radical scavenging, Fe3+ reducing and hepatic antilipid peroxidative activity of the complex were, respectively, 2.3-, 1.8- and 9.7-folds more potent than vanillic acid. Complexation increased the ci-glucosidase and glycation inhibitory activity of vanillic acid by 3- and 2.6-folds, respectively. Zn(II) conferred potent L-6 myotube (EC50 = 20.4 mu m) and muscle tissue (EC50 = 612 mu m) glucose uptake effects on vanillic acid. Cytotoxicity evaluation showed that the complex did not reduce the viability of L-6 myotubes and Chang liver cells. Conclusions The data suggest that Zn(II)-vanillic acid complex had improved bioactivity relative to vanillic acid.Thus, Zn(II) may be further studied as an antihyperglycaemic and anti-oxidative adjuvant for bioactive phenolic acids.

Automated summary

The study synthesized a new Zn-vanillic acid complex with enhanced antioxidative and antihyperglycaemic properties compared to vanillic acid alone. The complex demonstrated significant bioactivity in cell-based and ex vivo models.