期刊
JOURNAL OF PESTICIDE SCIENCE
卷 46, 期 3, 页码 258-266出版社
PESTICIDE SCI SOC JAPAN
DOI: 10.1584/jpestics.D21-021
关键词
chlorophyll fluorescence; Fe-chelatase; heme; photobleaching; porphyrin
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2018R1D1A1B07043814, NRF2020R1I1A3073932]
The expression of BjFeCh in transgenic rice helps alleviate NF-induced photobleaching by protecting the synthesis of chlorophyll intermediates and maintaining higher levels of porphyrin biosynthetic gene transcripts, contributing to understanding the resistance mechanism of NF-resistant crops and establishing a new weed control strategy.
We examined the effect of Bradyrhizobium japonicum FeCh (BjFeCh) expression on the regulation of porphyrin biosynthesis and resistance to norflurazon (NF)-induced photobleaching in transgenic rice. In response to NF, transgenic lines F4 and F7 showed lesser declines in chlorophyll, carotenoid, F-v/F-m, phi(PSII), and light-harvesting chlorophyll (Lhc) a/b-binding proteins as compared to wild-type ( WT) plants, resulting in the alleviation of NF-induced photobleaching. During photobleaching, levels of heme, protoporphyrin IX (Proto IX), Mg-Proto IX (monomethylester), and protochlorophyllide decreased in WT and transgenic plants, with lesser decreases in transgenic plants. Most porphyrin biosynthetic genes were greatly downregulated in WT and transgenic plants following NF treatment, with higher transcript levels in transgenic plants. The expression of BjFeCh in transgenic rice may play a protective role in mitigating NF-induced photobleaching by maintaining levels of heme, chlorophyll intermediates, and Lhc proteins. This finding will contribute to understanding the resistance mechanism of NF-resistant crops and establishing a new strategy for weed control.
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