期刊
JOURNAL OF PESTICIDE SCIENCE
卷 46, 期 1-2, 页码 206-213出版社
PESTICIDE SCI SOC JAPAN
DOI: 10.1584/jpestics.D21-003
关键词
flutianil; powdery mildew; haustorium; Blumeria graminis; microscopy; ultrastructure; RNA-seq
类别
Research suggests that flutianil primarily affects haustoria, impacting the morphology of powdery mildew fungi.
Flutianil, a fungicide effective only on powdery mildew, was previously reported to affect the host cell's haustorial formation and nutrient absorption. Studies were conducted to investigate flutianil's primary site of action on Blumeria graminis morphology using transmission electron microscope (TEM) observation and RNA sequencing (RAN-seq) techniques. TEM observation revealed that flutianil caused the extra-haustorial matrix and fungal cell wall to be obscured, without remarkable changes of other fungal organelles. RNA-seq analysis indicated that, unlike other powdery-mildew fungicides, flutianil did not significantly affect the constantly expressed genes for the survival of B. graminis. Genes whose expression is up- or downregulated by flutianil were found; these are the three sugar transporter genes and various effector genes, mainly expressed in haustoria. These findings indicate that the primary site of action of flutianil might be in the haustoria.
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