期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 86, 期 15, 页码 10819-10828出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.joc.1c01115
关键词
-
资金
- National Natural Science Foundation of China [22077130, 22007092]
- Shanghai Pujiang Program [20PJ1415800]
- Science and Technology Commission of Shanghai Municipality [20ZR1467900]
- Shanghai Municipal Science and Technology Major Project
- Shanghai Sailing Program [20YF1457200]
- State Key Laboratory of Medicinal Chemical Biology of Nankai University [2020013]
A diversity-oriented chemoenzymatic approach was used for the collective preparation of sulfated core 2 O-GalNAc glycans and their nonsulfated counterparts. A sulfated trisaccharide and a nonsulfated trisaccharide were chemically synthesized, then enzymatic extension was carried out using a panel of robust glycosyltransferases to achieve regioselective sialylation, resulting in the generation of 36 structurally well-defined O-GalNAc glycans.
A diversity-oriented chemoenzymatic approach for the collective preparation of sulfated core 2 O-GalNAc glycans and their nonsulfated counterparts was described. A sulfated trisaccharide and a nonsulfated trisaccharide were chemically synthesized by combining flexible protected group manipulations and sequential one-pot glycosylations. The divergent enzymatic extension of these two trisaccharides, using a panel of robust glycosyltransferases that can recognize sulfated substrates and differentiating the branches with specifically designed glycosylation sequences to achieve regioselective sialylation, provided 36 structurally well-defined O-GalNAc glycans.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据