期刊
JOURNAL OF NEUROSCIENCE
卷 41, 期 27, 页码 5822-5832出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0200-21.2021
关键词
TRP channels; TRPC; Drosophila; phototransduction; vision; electroretinogram
资金
- National Eye Institute [EY010852, 2P40OD010949]
This study identified the structural requirements for TRP channel function in Drosophila photoreceptor cells, including the roles of the S4-S5 linker and the TRP domain, as well as the differential requirements for two highly conserved motifs in the TRP domain for activation and protein stability. Genetic complementation experiments revealed an intrasubunit interaction between the S4-S5 linker and the S5 segment in Drosophila photoreceptor cells, which contributes to channel activation.
Transient receptor potential (TRP) channels are broadly required in animals for sensory physiology. To provide insights into regulatory mechanisms, the structures of many TRPs have been solved. This has led to new models, some of which have been tested in vitro. Here, using the classical TRP required for Drosophila visual transduction, we uncovered structural requirements for channel function in photoreceptor cells. Using a combination of molecular genetics, field recordings, protein expression analysis, and molecular modeling, we interrogated roles for the S4-S5 linker and the TRP domain, and revealed mutations in the S4-S5 linker that impair channel opening or closing. We also uncovered differential requirements for the two highly conserved motifs in the TRP domain for activation and protein stability. By performing genetic complementation, we found an intrasubunit interaction between the S4-S5 linker and the S5 segment that contributes to activation. This analysis highlights key structural requirements for TRP channel opening, closing, folding, and for intrasubunit interactions in a native context-Drosophila photoreceptor cells.
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