期刊
JOURNAL OF NEUROSCIENCE
卷 41, 期 40, 页码 8441-8459出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0860-21.2021
关键词
Basso mouse scale; disease-associated microglia; scRNAseq; spinal cord injury
资金
- Swedish Medical Research Council
- Swedish Society of Medicine
- Karolinska Institutet
- Swedish Brain Foundation
- Stockholm City Council
This study used single-cell RNA sequencing to investigate the temporal dynamics of immune cells harvested from the epicenter of traumatic spinal cord injury (SCI) induced in female mice. It was found that baseline microglia undergo permanent transcriptional reprogramming into a previously uncharacterized subtype of microglia with similarities to disease-associated microglia (DAM) and a distinct microglial state found during development. Depletion of microglia showed that DAM in SCI are derived from baseline microglia and enhance recovery of hindlimb locomotor function following injury.
Microglia are resident myeloid cells of the CNS. Recently, single-cell RNA sequencing (scRNAseq) has enabled description of a disease-associated microglia (DAM) with a role in neurodegeneration and demyelination. In this study, we use scRNAseq to investigate the temporal dynamics of immune cells harvested from the epicenter of traumatic spinal cord injury (SCI) induced in female mice. We find that as a consequence of SCI, baseline microglia undergo permanent transcriptional reprogramming into a previously uncharacterized subtype of microglia with striking similarities to previously reported DAM as well as a distinct microglial state found during development. Using a microglia depletion model we showed that DAM in SCI are derived from baseline microglia and strongly enhance recovery of hindlimb locomotor function following injury.
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