期刊
JOURNAL OF NEUROSCIENCE
卷 41, 期 33, 页码 7086-7102出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2849-20.2021
关键词
ex vivo; hippocampus; in vivo; Kir3/GirK; LTP/LTD; metaplasticity
资金
- Spanish Ministry of Economy and Competitiveness from MINECO-FEDER [BFU2017-82494-P]
- Fundacion Tatiana Perez de Guzman el Bueno
- Plan Propio de Investigacion Programmes of the University of Castilla-La Mancha
The G-protein-gated inwardly rectifying potassium (Kir3/GIRK) channel plays a crucial role in hippocampal-dependent cognitive functions. Pharmacological modulation of GIRK activity can affect neural excitability and synaptic plasticity in the hippocampus.
The G-protein-gated inwardly rectifying potassium (Kir3/GIRK) channel is the effector of many G-protein-coupled receptors (GPCRs). Its dysfunction has been linked to the pathophysiology of Down syndrome, Alzheimer's and Parkinson's diseases, psychiatric disorders, epilepsy, drug addiction, or alcoholism. In the hippocampus, GIRK channels decrease excitability of the cells and contribute to resting membrane potential and inhibitory neurotransmission. Here, to elucidate the role of GIRK channels activity in the maintenance of hippocampal-dependent cognitive functions, their involvement in controlling neuronal excitability at different levels of complexity was examined in C57BL/6 male mice. For that purpose, GIRK activity in the dorsal hippocampus CA3-CA1 synapse was pharmacologically modulated by two drugs: ML297, a GIRK channel opener, and Tertiapin-Q (TQ), a GIRK channel blocker. Ex vivo, using dorsal hippocampal slices, we studied the effect of pharmacological GIRK modulation on synaptic plasticity processes induced in CA1 by Schaffer collateral stimulation. In vivo, we performed acute intracerebroventricular (i.c.v.) injections of the two GIRK modulators to study their contribution to electrophysiological properties and synaptic plasticity of dorsal hippocampal CA3-CA1 synapse, and to learning and memory capabilities during hippocampal-dependent tasks. We found that pharmacological disruption of GIRK channel activity by i.c.v. injections, causing either function gain or function loss, induced learning and memory deficits by a mechanism involving neural excitability impairments and alterations in the induction and maintenance of longterm synaptic plasticity processes. These results support the contention that an accurate control of GIRK activity must take place in the hippocampus to sustain cognitive functions.
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