Expression of the foraging gene in adult Drosophila melanogaster

The foraging gene in Drosophila melanogaster, which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult foraging expression is less well characterized than in the larva. We characterized foraging expression in the brain, gastric system, and reproductive systems using a T2A-Gal4 gene-trap allele. In the brain, foraging expression appears to be restricted to multiple sub-types of glia. This glial-specific cellular localization of foraging was supported by single-cell transcriptomic atlases of the adult brain. foraging is extensively expressed in most cell types in the gastric and reproductive systems. We then mapped multiple cis-regulatory elements responsible for parts of the observed expression patterns by a nested cloned promoter-Gal4 analysis. The mapped cis-regulatory elements were consistently modular when comparing the larval and adult expression patterns. These new data using the T2A-Gal4 gene-trap and cloned foraging promoter fusion GAL4's are discussed with respect to previous work using an anti-FOR antibody, which we show here to be non-specific. Future studies of foraging's function will consider roles for glial subtypes and peripheral tissues (gastric and reproductive systems) in foraging's pleiotropic behavioral and physiological effects.

Automated summary

The foraging gene in Drosophila melanogaster plays multiple behavioral and physiological functions in both larval and adult flies. Its expression is characterized in the brain, gastric system, and reproductive systems, showing glial-specific cellular localization in the brain and widespread expression in gastric and reproductive systems. Multiple cis-regulatory elements responsible for observed expression patterns have been identified and shown to be modular when comparing larval and adult expression patterns. The study discusses the use of gene-trap and cloned foraging promoter fusion GAL4 methods, highlighting the importance of considering glial subtypes and peripheral tissues in understanding foraging's effects.