Deep eutectic solvents based assay for extraction and determination of zinc in fish and eel samples using FAAS

A new assay based on effective (high recovery) extraction by means of deep eutectic solvents (DESs) was developed for ppb level determination of zinc in fishes and eel samples. Choline chloride and Phenol in a 1:2 M ratio was selected as optimal DES-based extraction solvent. 8-Hydroxy quinoline was used as a chelating agent for zinc ions. The optimized conditions were found at pH value of 8, ligand concentration of 10 mg/L, THF volumetric ratio in bulk solution of 0.5:10. The total time at optimal sample preparation conditions was 5 min for digested sample. Developed method fits to the purpose of routine analysis of fishes and eels. The LOD and LOQ were found 0.041 mu g/kg and 0.136 mu g/kg with pre-concentration factor of 25 and RSD 1.7%. (c) 2021 The Authors. Published by Elsevier B.V.

Automated summary

A new assay utilizing deep eutectic solvents for efficient extraction was developed for ppb level determination of zinc in fish and eel samples, with optimized conditions achieving a quick and accurate result suited for routine analysis.