Nuclear-localized eukaryotic translation initiation factor 1A is involved in mouse preimplantation embryo development

Preimplantation embryo development is characterized by drastic nuclear reprogramming and dynamic stage-specific gene expression. Key regulators of this earliest developmental stage have not been revealed. In the present study, a non-classical nuclear-localization pattern of eIF1A was observed during early developmental stages of mouse preimplantation embryo before late-morula. In particular, eIF1A is most highly expressed in the nuclear of 2-cell embryo. Knockdown eIF1A by siRNA microinjection affected the development of mouse preimplantation embryo, resulted in decreased blastocyst formation rate. CDX2 protein expression level significantly down-regulated after eIF1A knockdown in morula stage. In addition, the mRNA expression level of Hsp70.1 was also decreased in 2-cell embryo. The results indicate an indispensable role of eIF1A in mouse preimplantation embryos.

Automated summary

This study revealed the important role of eIF1A in early mouse preimplantation embryo development. Knockdown of eIF1A affected blastocyst formation rate, down-regulated CDX2 protein expression level, and decreased Hsp70.1 mRNA expression level in 2-cell embryos.