期刊
JOURNAL OF MOLECULAR DIAGNOSTICS
卷 23, 期 8, 页码 986-999出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jmoldx.2021.05.011
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Transcriptomic analysis of Staphylococcus aureus in periprosthetic joint infection (PJI) revealed significant changes in gene expression related to biofilm formation, iron acquisition, and antimicrobial resistance, with no significant differences in expression between in vivo and in vitro conditions.
Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.
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