Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil

Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7(T) and R19(T), were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10-37 degrees C and pH 6.0-9.0, and in the presence of 0-1.5% (w/v) NaCl. Strain R7(T) contained iso-C-14:0, iso-C-15:0, iso-C-16:0, and summed feature 9 (comprising C-16:0 10-methyl and/or iso-C-17:1 omega 9c), whereas strain R19(T) contained iso-C-11:0 3-OH, C-16:1 omega 7c alcohol, iso-C-11:0, iso-C-15:0, iso-C-16:0, and summed feature 9 (comprising C-16:0 10-methyl and/or iso-C-17:1 omega 9c) as major cellular fatty acids (> 5%). Both strains contained ubiquin-one-8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7(T) and R19(T) calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7(T) and R19(T) were most closely related to Lysobacter panacisoli C8-1(T) and Lysobacter niabensis GH34-4(T) with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7(T) and R19(T) formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7(T) and R19(T) represent novel species of the genus Lysobacter, for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7(T) (= KACC 21663(T) = JCM 34257(T)) and R19(T) (= KACC 21767(T) = JCM 34258(T)), respectively.

Automated summary

Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria strains R7(T) and R19(T) isolated from sandy and forest soil in South Korea exhibited specific characteristics such as growth conditions, major cellular fatty acids, and enzymatic activities.