期刊
JOURNAL OF MEDICAL VIROLOGY
卷 93, 期 12, 页码 6833-6836出版社
WILEY
DOI: 10.1002/jmv.27240
关键词
COVID-19; E484K variant; melting curve analysis; R; 1 lineage; SARS-CoV-2
类别
资金
- JST-CREST [JPMJCR20H2]
- Japan Agency for Medical Research and Development [20nk0101612h0901]
This study conducted PCR testing on patients who visited the hospital between February and April 2021, revealing a rapid increase in cases with N501Y and E484K mutations from 8% to 46%, with these mutations being associated with R.1 lineage variants. However, there was no evidence of increased disease severity related to the R.1 variants.
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, such as B.1.1.7 and B.1.351, has become a crucial issue worldwide. Therefore, we began testing all patients with COVID-19 for the N501Y and E484K mutations by using polymerase chain reaction (PCR)-based methods. Nasopharyngeal swab samples from 108 patients who visited our hospital between February and April 2021 were analyzed. The samples were analyzed using reverse transcription-PCR with melting curve analysis to detect the N501Y and E484K mutations. A part of the samples was also subjected to whole-genome sequencing (WGS). Clinical parameters such as mortality and admission to the intensive care unit were analyzed to examine the association between increased disease severity and the E484K mutation. The ratio of cases showing the 501N + 484K mutation rapidly increased from 8% in February to 46% in March. WGS revealed that the viruses with 501N + 484K mutation are R.1 lineage variants. Evidence of increased disease severity related to the R.1 variants was not found. We found that the R.1 lineage variants rapidly prevailed in Tokyo in March 2021, which suggests the increased transmissibility of R.1 variants, while they showed no increased severity.
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