Spatial variation of picoplankton communities along a cascade reservoir system in Patagonia, Argentina

In this study we explored how picoplankton conununity structure and diversity varied along three cascade oligo-mesotrophic reservoirs of the Limay River (Patagonia, Argentina): Alicura, Piedra del Aguila and Ramos Mexia. We analyzed the spatial changes, covering lotic and lentic stretches along a gradient of 262 kin from Andes to steppe, and we also sampled the main affluent of the Limay River (Colton Cura). In all sampling sites the main limnological variables were measured, and the picoplankton abundance (autotrophic and heterotrophic) was analyzed by flow cytometry. The bacterial biodiversity was assessed using high throughput sequencing Illumina MiSeq. We expected an increase in the trophic state along this series of cascade reservoirs, which would determine spatial differences in the structure of the picoplankton communities. We also hypothesized that the lotic and lentic conditions along the system would influence the bacterial composition. The results showed a slight increase in trophic state together with an increase in overall picoplankton abundance downstream, towards Ramos Mexia Reservoir. Picocyanobacteria were represented by phycoerythrin-rich cells all along the system, in accordance to the pattern described for oligotrophic aquatic ecosystems. Multivariate analyses based on bacterial OTU composition and environmental variables showed a spatial ordination of sites following the trend of increasing trophic state downstream. Molecular analyses of bacterial OTU diversity also showed an increase in richness and a decrease in evenness at the lotic stretches, and the opposite pattern in the reservoirs, suggesting that water retention time may play a role in structuring the community composition.

Automated summary

This study investigated how picoplankton community structure and diversity changed along three cascade reservoirs of the Limay River in Patagonia, Argentina. Results showed a slight increase in trophic state and picoplankton abundance downstream. Picocyanobacteria were consistently present with phycoerythrin-rich cells. Multivariate analyses revealed spatial ordination following the trend of increasing trophic state downstream. Molecular analyses indicated differences in bacterial OTU richness and evenness between lotic stretches and reservoirs.