期刊
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
卷 49, 期 9, 页码 -出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/03000605211036845
关键词
NLRP3; hepatitis B virus; liver failure; inflammasome; CD40-CD40L; case-control study
This study found significant activation of the NLRP3 inflammasome in patients with HBV-ACLF, which mediates liver failure by stimulating procaspase-1 and pro-IL-1 beta, as well as regulating downstream CD40-CD40L signaling.
Objectives In this prospective case-control study, we explored the regulatory roles of the NLRP3 inflammasome in hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF). Methods Thirty patients with HBV-ACLF, 30 patients with chronic hepatitis B, and 30 healthy individuals were enrolled. Real-time reverse transcription polymerase chain reaction was used to assess mRNA levels in peripheral blood mononuclear cells and serum protein levels were assessed by enzyme-linked immunosorbent assay. Results Serum levels of alanine aminotransferase, asparagine aminotransferase, total bilirubin, and direct bilirubin in patients with HBV-ACLF were increased. Transcript levels of NLRP3 and ASC and protein levels of interleukin (IL)-1 beta, IL-18, and sCD40L were elevated in patients with HBV-ACLF. Expression of the NLRP3 inflammasome signaling pathway components procaspase-1 and pro-IL-1 beta was elevated in patients with HBV-ACLF. Conclusions This prospective case-control study demonstrated that significant activation of the NLRP3 inflammasome occurs in patients with HBV-ACLF. The activated NLRP3 inflammasome mediated liver failure by stimulating procaspase-1 and pro-IL-1 beta and regulating downstream CD40-CD40L signaling.
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