期刊
FOOD RESEARCH INTERNATIONAL
卷 86, 期 -, 页码 9-23出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2016.05.013
关键词
Smooth hound by-products; Simultaneous protein hydrolysis; Antioxidant activity; Antibacterial activity; ACE inhibitory activity; RP-HPLC analysis; Molecular weight distribution
资金
- Ministry of Higher Education and Scientific Research, Tunisia
- Emerging Research Group Grant from Generalitat Valenciana in Spain [GV/2015/138]
- JAEDOC-CSIC post-doctoral contract
The aim of this study was to investigate the antioxidant, anti-ACE and antibacterial activities of smooth hound viscera hydrolysates (SHVHs). Viscera, as by-product, were hydrolyzed with crude alkaline protease extract from the same species, three commercial proteases and a combination endogenous and exogenous preparations. Hydrolysates showed different degrees of hydrolysis and the digestion with endogenous enzymes in combination with commercial proteases was found to enhance protein hydrolysis. The resulting SHVHs had high protein content (70.89%-89.06%) and they were mainly constituted by Gly, Glu and Gln, while Tau was the major free amino acid. In addition, they contained high amounts of UMP, Uridine, GMP and Guanosine, while undigested proteins were mainly rich in IMP and Xanthine. Furthermore, SHVHs showed different molecular mass distribution and RP-HPLC profiles proving their molecular mass and hydrophilic/hydrophobic peptide heterogeneity. All the SHVHs exhibited antioxidant activity, in terms of radical-scavenging activity, reducing power, metal chelating activity, beta-carotene protection, lipid peroxidation inhibition and DNA breakage assay, and it was found that a medium degree of hydrolysis was appropriate to obtain hydrolysates with good antioxidant capacity. In addition, the SHVHs antioxidant activity was improved after simulated gastrointestinal digestion. Besides their antibacterial effect against several Gram-positive and Gram-negative bacteria, all the hydrolysates showed varying degrees of ACE inhibitory activities and the highest one was achieved by the Purafect hydrolysate (IC50 = 75 mu g/mL). The overall data suggested that the SHVHs could be used as potential source of natural antioxidant, antimicrobial and anti-ACE peptides to formulate functional foods. (C) 2016 Elsevier Ltd. All rights reserved.
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