Novel Green Synthesis of Zinc Oxide Nanoparticles Using Orange Waste and Its Thermal and Antibacterial Activity

Zinc oxide nanoparticles (ZnO NPs) prepared via eco-friendly synthesis (using orange peels) are investigated. Pseudomonas Aeruginosa as a gram negative bacteria and Bacillus subtilis and Staphylococcus Aureus as a gram positive bacteria are used for studying antibacterial activity. XRD, FTIR-ATR and UV-Vis data confirmed the formation of ZnO NPs. Also, from XRD, UV-Vis data, optical band gap and HRTEM affirmed that the size of ZnO NPs prepared by chemical method is greater than that green synthesis methods. TGA and DTG indicates the high thermal stability of ZnO NPs prepared by chemical method than that prepared to green synthesis method. Antibacterial effect showed the slightly superior of the ZnO prepared by chemical method on the Prepared by green method at levels 50 and 100 ppm, while no differences found at 150 ppm in the Pseudomonas aeruginosa and B. subtilis. On the other hand, the comparable results for two zinc oxide nanoparticles in the S. aureus.

Automated summary

In this study, zinc oxide nanoparticles prepared using orange peels were investigated for antibacterial activity against various bacteria. The results showed that nanoparticles prepared using chemical methods were larger in size and exhibited higher thermal stability compared to those prepared using green synthesis methods. The antibacterial effect of the nanoparticles differed slightly at lower concentrations, but showed comparable results at higher concentrations, depending on the type of bacteria tested.