期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 424, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.127251
关键词
Dissolved biochar; Copper ion; Antibiotic resistance genes; Conjugative transfer; RP4 plasmid
资金
- National Natural ScienceFoundation of China [42007228, U1806216, 41877372]
- Na-tional Key R&D Program of China [2018YFC1802002]
- 111 program, Ministry of Education, China [T2017002]
The addition of dissolved biochar effectively weakened or eliminated the Cu(II)-facilitated efficient transfer of ARGs. The transfer efficiency was entirely inhibited when the Cu(II) concentration exceeded 0.5 mg/L. Factors such as complex formation between Cu(II) and humic acidlike components and promotion of low molecule organic matters dominated the transfer efficiency.
The proliferation of antibiotic resistance genes (ARGs) has posed significant risks to human and environmental health. Research has confirmed that Cu(II) could accelerate the conjugative transfer of ARGs between bacteria. This study found that adding dissolved biochar effectively weakened or eliminated the Cu(II)-facilitated efficient transfer of ARGs. The efficiency of conjugative transfer was promoted after treatment with Cu(II) (0.05 mg/L) or dissolved biochar at a pyrolysis temperature of 300 degrees C. When exposed to the combination of Cu(II) and dissolved biochar, the transfer frequency was significantly reduced; this occurred regardless of the Cu(II) concentration or pyrolysis temperature of dissolved biochar. In particular, when the Cu(II) concentration exceeded 0.5 mg/L, the transfer efficiency was entirely inhibited. Gene expression analysis indicated that different treatments affect transfer efficiency by regulating the expression of three global regulatory genes: korA, korB, and trbA. Among them, humic acid repressed the expression of these genes; however, Cu(II) formed complex with the humic acidlike components, gradually weakening the inhibitive effect of these components. The promotion of low molecule organic matters dominated, resulting in a dynamic decline in the transfer efficiency. This study provides a new environmental contaminant treatment approach to eliminate the heavy metal-facilitated transfer of ARGs between bacteria.
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