4.7 Article

Sensitive and label-free chemiluminescence detection of malathion using exonuclease-assisted dual signal amplification and G-quadruplex/ hemin DNAzyme

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 411, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124784

关键词

Malathion; Aptasensor; Dual signal amplification; G-quadruplex DNAzyme; Traditional Chinese medicine

资金

  1. National Natural Science Foundation of China [81771905]
  2. Traditional Chinese Medicine Technology Project of Wuxi Administration of Traditional Chinese Medicine [ZYKJ201903, ZYKJ202001]
  3. Wuxi Science and Technology Development Fund Project [N20202039]
  4. Jiangsu Provincial Key Medical Discipline [ZDXKA2016017]
  5. Innovation Capacity Development Plan of Jiangsu Province [BM2018023]

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This study demonstrated a label-free chemiluminescent aptasensor for sensitive detection of malathion using exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme. The aptasensor showed an excellent linear response to malathion with a detection limit of 0.47 pM under optimal conditions, and successfully detected malathion in spiked food and traditional Chinese medicine samples.
Malathion is one of the most commonly used organophosphorus pesticides that can cause serious harm to the ecological environment and human health. Herein, we demonstrated a label-free chemiluminescent aptasensor for the sensitive detection of malathion based on exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme. Upon the addition of malathion, the aptamer probe specifically bound to the target to form a complex malathion-S3, leaving a duplex S1-S2. The complex malathion-S3 was digested by exonuclease I and the target was released. The released target was recycled to perform exonuclease I-assisted signal amplification. Furthermore, after treatment with exonuclease III, the duplex S1-S2 was converted into the secondary target ST. The secondary target ST interacted with the hairpin H1 to form a complex H1-ST, which was further digested by exonuclease III and released the secondary target. The released secondary target was recycled to perform exonuclease III-assisted signal amplification. After complete amplification, large numbers of G-quadruplex/hemin DNAzymes were generated. Under the optimal experimental conditions, the prepared aptasensor showed an excellent linear response to malathion with a detection limit of 0.47 pM. The relative standard deviations were in the range of 4.2?6.9%. Moreover, the aptasensor was successfully applied to detect malathion in spiked food and traditional Chinese medicine samples.

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