Loop-mediated isothermal amplification of bacterial effector genes to detect Pseudomonas syringae pv. actinidiae biovars 1 and 3

Loop-mediated isothermal amplification (LAMP) was designed to rapidly detect biovars 1 and 3 of Pseudomonas syringae pv. actinidiae (Psa), a serious, global kiwifruit pathogen, using two primer sets targeting biovar-specific type III effector genes. Each primer set was specific for the targeted biovars, and no signals were obtained for any other biovars. Detection limits of the assay were tenfold higher than that of the conventional PCR method for both biovars. The primer set to detect biovar 3 was highly sensitive at detecting this biovar directly from leaf lesions.

Automated summary

LAMP technology was developed for rapid detection of biovars 1 and 3 of Pseudomonas syringae pv. actinidiae (Psa), offering higher detection limits compared to conventional PCR. The primer set for biovar 3 showed high sensitivity in directly detecting this biovar from leaf lesions.