期刊
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
卷 102, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2021.103992
关键词
Aggregation; Aptamer; Dairy product; Enzyme-free; Mycotoxin; Rapid detection
资金
- Agricultural Research Development Agency (Public Organization) Thailand (ARDA)
- Thailand Science Research and Innovation (TSRI)
- Veterinary Research and Development Center (Upper Northern Region) Livestock Division, Thailand
- Chiang Mai University
- Center of Excellence for Innovation in Chemistry
The development of a label-free colorimetric aptasensor for rapid quantification of aflatoxin M1 (AFM1) has been accomplished based on localized surface plasmon resonance (LSPR) and aggregation of gold nanoparticles (AuNPs). The sensor has a detection limit of 0.002 ng mL-1, high analytical sensitivity, and practicality for real samples.
The development of a label-free colorimetric aptasensor has been accomplished for rapid quantification of aflatoxin M1 (AFM1) based on localized surface plasmon resonance (LSPR) and aggregation of gold nanoparticles (AuNPs). The sensor utilizes competitive interactions of the aptamer to the AFM1 or the AuNPs under a specific condition exploiting sodium chloride to aggregate AuNPs. In principle, the aptamer interacts with AFM1 selectively, resulting in a change of its structure; consequently, the aptamer cannot prevent AuNPs aggregation induced by sodium chloride. A new color tone of the solution caused by the LSPR peak shift of aggregated AuNPs can be measured by a colorimetric method. The detection limit of the sensor was 0.002 ng mL-1, providing exceptionally analytical sensitivity that conforms to the maximal residue limits of AFM1. Moreover, the current method has been successfully utilized to determine AFM1 in milk. Recoveries obtained were higher than 80 %, with RSD lower than 10 %, denominating that the colorimetric aptasensor devotes practicability for real samples.
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