Gibberellic acid maintains postharvest quality of Agaricus bisporus mushroom by enhancing antioxidative system and hydrogen sulfide synthesis

The application of gibberellic acid (GA(3)) treatment to the postharvest quality maintenance of white button mushroom (Agaricus bisporus) was investigated. The optimum concentration of exogenous GA(3) was 100 mg/L. At this concentration, the color change was inhibited, the firmness was maintained, and the weight loss and respiratory rates were reduced. The GA(3) group had significantly lower malonaldehyde (MDA) content and membrane permeability. Reactive oxygen species accumulation was reduced due to the regulation of polyphenol oxidase (PPO), peroxidase (POD), and superoxide dismutase (SOD) enzyme activities. Moreover, the production of endogenous gaseous signaling molecule hydrogen sulfide (H2S) was triggered by GA(3) treatment, which enhanced cystathionine gamma-lyase (AbCSE) and cystathionine beta-synthase (AbCBS) activities alongside the corresponding gene expressions. The preservation of button mushroom postharvest storage quality by GA(3) was most likely due to the regulation of reactive oxygen species metabolism and hydrogen sulfide biosynthesis. Practical applications Mushroom is rich in nutrients and functional substances. However, due to the lack of cuticle, high respiration rate, and moisture content, mushroom's postharvest quality deteriorates rapidly. A safe and effective reagent that prevents the senescence and quality deterioration of harvested mushroom is urgently needed. The effects of plant hormone GA(3) on the postharvest quality of edible fungi remain unclear. The present study provided convincing evidence that 100 mg L-1 of GA(3) effectively maintained postharvest button mushroom quality by regulating reactive oxygen species metabolism and hydrogen sulfide biosynthesis.

Automated summary

The study found that treatment with 100 mg/L gibberellic acid (GA(3)) effectively maintained the postharvest quality of white button mushrooms by regulating reactive oxygen species metabolism and hydrogen sulfide biosynthesis.