期刊
JOURNAL OF ENVIRONMENTAL SCIENCES
卷 107, 期 -, 页码 218-229出版社
SCIENCE PRESS
DOI: 10.1016/j.jes.2021.01.029
关键词
COVID-19; Wastewater surveillance; Public health; Quality assurance; Quality control
资金
- CHEO (Children's Hospital of Eastern Ontario) CHAMO (Children's Hospital Academic Medical Organization) grant
- Next generation solutions to ensure healthy water resources for future generations - Global Water Futures program, Canada First Research Excellence Fund [419205]
- Canada Research Chairs Program of the Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC
- NSERC Alliance COVID-19 Grant
- Mitacs through the Mitacs Accelerate program
- Canadian Institutes of Health Research (CIHR), Alberta Innovates, Alberta Health-Water for Life Strategy
- BC center for Disease Control
- BC center for Disease Control Foundation for Public Health and Metro Vancouver
- Canada Research Chairs Program of NSERC
Detection of SARS-CoV-2 RNA in wastewater can inform public health decisions during the COVID-19 pandemic, but the analysis methods are not yet standardized globally. Research in Canada showed that different methods yielded comparable results with low variability, suggesting the need for a consistent method for evaluating wastewater SARS-CoV-2 temporal trends.
Detection of SARS-CoV-2 RNA in wastewater is a promising tool for informing public health decisions during the COVID-19 pandemic. However, approaches for its analysis by use of reverse transcription quantitative polymerase chain reaction (RT-qPCR) are still far from standardized globally. To characterize inter- and intra-laboratory variability among results when using various methods deployed across Canada, aliquots from a real wastewater sample were spiked with surrogates of SARS-CoV-2 (gamma-radiation inactivated SARS-CoV-2 and human coronavirus strain 229E [HCoV-229E]) at low and high levels then provided blind to eight laboratories. Concentration estimates reported by individual laboratories were consistently within a 1.0-log(10) range for aliquots of the same spiked condition. All laboratories distinguished between low- and high-spikes for both surrogates. As expected, greater variability was observed in the results amongst laboratories than within individual laboratories, but SARS-CoV-2 RNA concentration estimates for each spiked condition remained mostly within 1.0-log(10) ranges. The no-spike wastewater aliquots provided yielded non-detects or trace levels (<20 gene copies/mL) of SARS-CoV-2 RNA. Detections appear linked to methods that included or focused on the solids fraction of the wastewater matrix and might represent in-situ SARS-CoV-2 to the wastewater sample. HCoV-229E RNA was not detected in the no-spike aliquots. Overall, all methods yielded comparable results at the conditions tested. Partitioning behavior of SARS-CoV-2 and spiked surrogates in wastewater should be considered to evaluate method effectiveness. A consistent method and laboratory to explore wastewater SARS-CoV-2 temporal trends for a given system, with appropriate quality control protocols and documented in adequate detail should succeed. (c) 2021 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
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