期刊
JOURNAL OF ENVIRONMENTAL MANAGEMENT
卷 288, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jenvman.2021.112379
关键词
Stoichiometric imbalance; Ecological stoichiometry; Microbial nutrient limitation; Gene abundance; Greenhouse vegetable cropping; Nitrogen cycle
资金
- National Natural Science Foundation of China [41977078]
Partially substituting chemical fertilizer with organic fertilizer in intensive greenhouse vegetable ecosystem can aggravate microbial N limitation and affect N-cycling gene abundances. Organic substitution can improve crop yield and reduce microbial N limitation.
Partially substituting chemical fertilizer with organic fertilizer has substantially changed the stoichiometric imbalances of carbon (C), nitrogen (N) and phosphorus (P) between microbial communities and their available resources in agroecosystems. However, how organic substitution alters microbial nutrient limitation and then affects soil N cycle in intensive greenhouse vegetable ecosystem, remain unknown. Thus, we performed a threeyear greenhouse vegetable field experiment in China with different fertilization strategies: no N fertilization, chemical N fertilization, and substituting 20% (1M4N) or 50% (1M1N) of chemical N with organic fertilizer (organic substitutions). Our results demonstrated that the microbial communities presented N limitation, accompanying with a strong N:P but a weak C:N (or P) microbial homeostasis in response to high N:P imbalance among all treatments. Organic substitutions at 1M1N and 1M4N significantly aggravated microbial N limitation and decreased the gene abundances of nitrification and denitrification by 4.7%?27.3% than that of chemical N fertilization. Microbial N limitation was strongly influenced by N:P stoichiometric imbalance illustrated from regression analysis. The N-cycling gene abundances were not only dependent on the inorganic N pool and soil physicochemical properties (i.e. pH and electrical conductivity), but also affected by microbial nutrient limitation inferred from random forest analysis. Furthermore, the 1M1N treatment performed better than the 1M4N in terms of improved crop yield and less microbial N limitation. Overall, these results highlight the importance of ecological stoichiometry in regulating soil N cycle under different fertilization strategies for intensive greenhouse vegetable ecosystem.
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