期刊
FOOD CHEMISTRY
卷 192, 期 -, 页码 163-170出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.06.086
关键词
Aqueous two-phase system; Polyoxyethylene lauryl ether; Trace; Chloramphenicol; Determine
资金
- National Natural Science Foundation of China [31470434, 21406090, 21206059, 21407058]
- Natural Science Foundation of Jiangsu Province [BK20141289, BK20131258]
- China Postdoctoral Science Foundation [2013M531284]
- Natural Science Foundation of Jilin Province [20150520062JH, 20140101206JC, 20130101179JC_15]
- Science and Technology Research Foundation of Jilin Province Department of Education [2014_158]
Polyoxyethylene lauryl ether (POELE10)-NaH2PO4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP (E%) is up to 99.42% under the optimal conditions, namely, the concentration of NaH2PO4, the concentration of POELE10, pH and temperature were 0.186 g.mL(-1), 0.033 g.mL(-1), 3.8 and 25 degrees C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH2PO4 was 0.192 g.mL(-1), the concentration of POELE10 was 0.024 g/ml, pH was 4.2 and temperature was 30 C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8 mu g.kg(-1) and 1 mu g.kg(-1), which meet the needs of determining trace or ultratrace CAP in food. The E% and F of this technique are much better than other extraction methods. (C) 2015 Elsevier Ltd. All rights reserved.
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