期刊
FOOD CHEMISTRY
卷 200, 期 -, 页码 32-37出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.01.001
关键词
Magnetic molecularly imprinted polymers; Malachite green; Aquatic products; High-performance liquid chromatography
资金
- Foundation for Innovative Research Team of Jimei University, China [2010A007]
- SRF for ROCS, SEM [201210100002]
- Science and Technology Planning Project of Fujian Province, China [2012Y0052, 2014Y0045]
- Natural Science Foundation of Fujian Province of China [2015J01615]
- Science and Technology Planning Project of Xiamen, China [3502Z20143018]
Magnetic molecularly imprinted polymers (MMIPs) were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid as monomer, ethylene dimethacrylate as crosslinker, and Fe3O4 magnetite as magnetic component. MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometry. Under the optimum condition, the MMIPs obtained exhibited quick binding kinetics and high affinity to MG in the solution. Scatchard plot analysis revealed that the MMIPs contained only one type of binding site with dissociation constant of 24.0 mu g mL (1). The selectivity experiment confirmed that the MMIPs exhibited higher selective binding capacity for MG than its structurally related compound (e.g., crystal violet). As a sorbent for the extraction of MG in sample preparation, MMIPs together with the absorbed analytes could easily be separated from the sample matrix with an external magnet. After elution with methanol/acetic acid (9: 1, v/v), MG in the eluent was determined by high-performance liquid chromatography coupled with UV detector with recoveries of 94.0-115%. Results indicated that the as-prepared MMIPs are promising materials for MG analysis in aquatic products. (C) 2016 Elsevier Ltd. All rights reserved.
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