Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern

Article Microbiology

Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19

Ahmed Babiker et al.

Summary: Our study found that current broad-spectrum molecular testing algorithms can identify most respiratory viral infections among SARS-CoV-2 PUIs when available and implemented consistently.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Infectious Diseases

Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020

Antonin Bal et al.

Summary: The study reported the strategy leading to the first detection of the variant of concern 202012/01 (VOC) in France, by screening for mutations such as Delta H69/Delta V70 associated with VOC and identifying co-occurring mutations like S:N501Y.

EUROSURVEILLANCE (2021)

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Review Microbiology

The Importance and Challenges of Identifying SARS-CoV-2 Reinfections

Ahmed Babiker et al.

Summary: Reports of SARS-CoV-2 reinfection have raised questions about the immune response and the possibility of partial immune protection. Further research is needed to elucidate the frequency, determinants, and consequences of these reinfections. Collaborative efforts involving clinical laboratories and investigators are crucial to establish frameworks for surveillance and investigation.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Public, Environmental & Occupational Health

Emergence of SARS-CoV-2 B.1.1.7 Lineage — United States, December 29, 2020–January 12, 2021

Summer E. Galloway et al.

MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT (2021)

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Article Medicine, General & Internal

Genomic sequencing in pandemics

The Lancet

LANCET (2021)

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Article Multidisciplinary Sciences

Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccine-elicited antibodies

Dami A. Collier et al.

Summary: The B.1.1.7 variant of SARS-CoV-2 exhibited reduced neutralization by vaccines and antibodies from recovered COVID-19 patients, with a more substantial loss seen when introducing the E484K mutation. This mutation poses a threat to the efficacy of the BNT162b2 vaccine.

NATURE (2021)

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Article Multidisciplinary Sciences

Detection of a SARS-CoV-2 variant of concern in South Africa

Houriiyah Tegally et al.

Summary: The article describes a newly emerged lineage of SARS-CoV-2, 501Y.V2, characterized by eight mutations in the spike protein, which may result in increased transmissibility or immune escape. This lineage originated in South Africa and quickly became dominant in Eastern Cape, Western Cape, and KwaZuluNatal provinces within weeks.

NATURE (2021)

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Article Multidisciplinary Sciences

Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England

Nicholas G. Davies et al.

Summary: A new variant of SARS-CoV-2 has emerged in England, with a higher reproduction number and potential for large resurgences of COVID-19 cases. Without stringent control measures, it is projected that COVID-19 hospitalizations and deaths in England for the first 6 months of 2021 will exceed those in 2020. The variant has spread globally and exhibited similar transmission increases in Denmark, Switzerland, and the United States.

SCIENCE (2021)

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Article Microbiology

Infection- and vaccine-induced antibody binding and neutralization of the B.1.351 SARS-CoV-2 variant

Venkata Viswanadh Edara et al.

Summary: Despite reduced antibody titers against the B.1.351 variant, sera from infected and vaccinated individuals containing polyclonal antibodies to the spike protein could still neutralize SARS-CoV-2 B.1.351, suggesting that protective humoral immunity may be retained against this variant.

CELL HOST & MICROBE (2021)

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Article Biochemistry & Molecular Biology

Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2

Chantal B. F. Vogels et al.

Summary: With the emergence of SARS-CoV-2 variants with increased transmissibility and immune escape, targeted surveillance is urgently needed. The B.1.1.7 variant can be detected by a specific deletion in the spike gene, but other variants like B.1.351 and P.1 cannot. An open-source PCR assay targeting a deletion in the ORF1a gene has been designed and validated to enhance surveillance for these variants.

PLOS BIOLOGY (2021)

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Article Microbiology

Multiplex SARS-CoV-2 Genotyping Reverse Transcriptase PCR for Population-Level Variant Screening and Epidemiologic Surveillance

Hannah Wang et al.

Summary: This study developed a new nucleic acid amplification test to detect SARS-CoV-2 variants with high sensitivity and specificity. Screening a large cohort of infected individuals in the San Francisco Bay Area revealed a rapid increase in the prevalence of the L452R variant over time.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Immunology

Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

Marek Widera et al.

Summary: The study demonstrates that bamlanivimab, casirivimab, and imdevimab can efficiently neutralize authentic SARS-CoV-2, but some variants may be resistant to these monoclonal antibodies.

JOURNAL OF INFECTIOUS DISEASES (2021)

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Letter Infectious Diseases