期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2021.122919
关键词
Chenopodium quinoa Willd; alpha-glucosidase inhibitors; Surface plasmon resonance; Liquid chromatography-mass spectrometry; Molecular docking analysis
资金
- National Natural Science Foundation [318703333, 31800297]
- Qinghai Provincial Science Foundation [2019-ZJ-7100]
- Key R&D and Transformation Plan of Xining [2018-Y-15]
- Youth Innovation Promotion Association, CAS, Qinghai Province High-end Innovative Thousand Talents Program [2021-ZJ-T05]
- CAS Light of West China Program
- Youth Innovation Promotion Association, CAS, Qinghai Province Innovation Plat-form for the Development [2021-ZJ-T05]
This study established a method for rapid identification of glycosidase inhibiting compounds from quinoa bran, and identified a variety of flavonoids and triterpenoid saponins that may bind to alpha-glycosidase using SPR and LC-MS. Analysis of interaction kinetics and molecular docking supported their alpha-glucosidase-inhibiting activity, indicating the potential of quinoa bran as a natural source of such inhibitors.
Extracts from the bran of Chenopodium quinoa Willd. (QBE) were reported to be active in inhibiting alpha-glycosidase, a promising target for treatment of diabetes mellitus. However, the constituents responsible for the alpha-glucosidase-inhibiting activity of QBE have not been fully characterized. The present study aimed to set up a method for rapid identification of glycosidase inhibiting compounds from the quinoa bran. With surface plasmon resonance (SPR) coupled with liquid chromatography-mass spectrometry (LC-MS), we identified eight flavonoids and ten triterpenoid saponins that may bind to the alpha-glycosidase. Analysis of the interaction kinetics by molecular docking supported their alpha-glucosidase-inhibiting activity and revealed the potential mechanisms for the inhibitory effects. In summary, this study established a SPR and LC-MS-based method for rapid in vitro screening of alpha-glucosidase inhibitors and suggested the quinoa bran a potential natural source of alpha-glucosidase inhibitors.
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