4.6 Article

Preparative separation of structural isomeric pentacyclic triterpenes from Eriobotrya japonica (Thunb.) leaves by high speed countercurrent chromatography with hydroxypropyl-β-cyclodextrin as additive

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JOURNAL OF CHROMATOGRAPHY A
卷 1646, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.chroma.2021.462066

关键词

High speed countercurrent chromatography; Maslinic acid; Corosolic acid; Hydroxypropyl-beta-cyclodextrin; Eriobotrya japonica

资金

  1. National Natural Science Foundation of China [82073979]
  2. Zhejiang Provincial Natural Science Foundation of China [LY20H290009]

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Maslinic acid and corosolic acid were successfully separated from Eriobotrya japonica leaves with high purity using a two-step countercurrent chromatographic separation technique. Two biphasic solvent systems were selected based on the partition performance of the main structural isomeric pentacyclic triterpenes. The recoveries of the two isomers were over 90%, demonstrating the effectiveness of the separation method.
Maslinic acid and corosolic acid with high purity were successfully separated from Eriobotrya japonica (Thunb.) leaves by two-step countercurrent chromatographic separation. Two biphasic solvent systems composed of petroleum ether-ethyl acetate-ethanol-water (6:4:5:5, v/v) and petroleum ether-ethyl acetate-ethanol-0.10 mol/L of hydroxypropyl-beta-cyclodextrin with pH 7.0 (8:2:3.5:6.5, v/v) were selected according to the partition performance of the main structural isomeric pentacyclic triterpenes. The influences of pH value and concentration of hydroxypropyl-beta-cyclodextrin in separation of two isomers were investigated. In first step countercurrent chromatographic separation, a mixture of two target structural isomers (14.12 mg of sample I) was separated from 40.00 mg of a partially purified sample. In second step countercurrent chromatographic separation, maslinic acid and corosolic acid were completely isolated from 12.00 mg of sample I with hydroxypropyl-beta-cyclodextrin as aqueous phase additive. The recoveries of the two isomers were over 90%, yielding 5.18 mg of maslinic acid and 5.47 mg of corosolic acid, respectively. (C) 2021ElsevierB.V. Allrightsreserved.

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