期刊
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
卷 97, 期 9, 页码 2376-2385出版社
WILEY
DOI: 10.1002/jctb.6821
关键词
continuous bioprocessing; virus-like particles; multicolumn chromatography; continuous filtration; process integration
类别
资金
- Department of Biotechnology, Ministry of Science and Technology [BT/COE/34/SP15097/2015]
- University of Queensland-IIT Delhi Academy of Research (UQIDAR)
Virus-like particles (VLPs) have great potential as vaccines, but face challenges in production. Continuous bioprocessing offers new opportunities for manufacturing VLPs. The biopharmaceutical industry is gradually shifting towards continuous production.
Virus-like particles (VLPs) are bioengineered protein complexes that can serve as prophylactic vaccines due to favorable immunological characteristics like size, repetitive surface geometry and ability to induce both innate and adaptive immune responses, without being infectious. Large-scale manufacturing of VLPs presents some interesting and unique challenges associated with removal of refractory contaminants to attain high product purity, improvement in structural homogeneity and colloidal stability. Recent advancements in production and purification of VLP-based vaccines, such as application of monolithic multicolumn chromatography and inline concentration and diafiltration, have set a stage for implementation of continuous bioprocessing for commercial manufacturing of VLPs. The benefits of continuous bioprocessing have been well demonstrated for biotherapeutics, particularly monoclonal antibodies and small peptides. With the growing demand for affordable vaccines, the biopharmaceutical industry has shown increasing interest in establishing end-to-end continuous platforms. This article reviews major developments that have occurred in continuous processing for the manufacturing of VLPs. The major problems inherent in transitions from batch to continuous processing are also discussed, along with potential strategies to overcome the challenges. (c) 2021 Society of Chemical Industry (SCI).
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