4.7 Article

Molecular Mechanism of Ca2+in the Allosteric Regulation of Human Parathyroid Hormone Receptor-1

期刊

JOURNAL OF CHEMICAL INFORMATION AND MODELING
卷 62, 期 21, 页码 5110-5119

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jcim.1c00471

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资金

  1. National Natural Science Foundation of China
  2. Post-graduate Research & Practice Innovation Program of Jiangsu Province
  3. [21605041]
  4. [KYCX21_0568]

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Extensive molecular dynamics simulations of PTH1R-PTH complexes reveal that Ca2+ ions play a crucial role in regulating PTH1R activation through interactions with specific acidic residues, inducing protein conformational changes, and enhancing residue-residue communication.
Parathyroid hormone (PTH) is an endogenous ligand that activates the PTH type 1 receptor (PTH1R) signaling. Ca2+, a common second messenger, acts as an allosteric regulator for prolonging the activation of PTH1R. However, a clear picture of the underlying allosteric mechanism is still missing. Herein, extensive molecular dynamics (MD) simulations are performed for PTH1R-PTH complexes with and without Ca2+ ions, allowing us to delineate the molecular details of calcium-induced allostery. Our results indicate that acidic residues in the extracellular loop 1 (ECL1) (D251, E252, E254, and E258-E260) and PTH (E19 and E22) serve as key determinants for local Ca2+- coupling structures and rigidity of ECL1. Moreover, the binding of Ca2+ induces conformational changes of transmembrane domain 6/7 (TM6/7) that are related to PTH1R activation and strengthens the residue-residue communication within PTH and TMD allosterically. Moreover, our results demonstrate that the presence of Ca2+ ions potentiates the interaction between PTH and PTH1R via steered molecular dynamics (SMD) simulations, while the point mutation in the PTH (PTHR25C) weakens the binding of PTH and PTH1R. These results support that Ca2+ ions might further prolong the residence time of PTH on PTH1R and facilitate the positive allostery of PTH1R. Together, the present work provides new insights into the allosteric regulation mechanism of GPCRs induced by ions and related drug design targeting the PTH1R allosteric pathway.

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