期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 122, 期 10, 页码 1413-1427出版社
WILEY
DOI: 10.1002/jcb.30018
关键词
adenosine 3 ',5 '-cyclic monophosphate; adenylate cyclase; Leishmania donovani; oxidative stress; protein kinase A; reactive oxygen species
资金
- Indian Council of Medical Research [INT 133/BAS/2017]
- Science and Engineering Research Board [SB/S2/JCB-020/2016]
- Department of Biotechnology, Ministry of Science and Technology [DBT/JRF/16/AL/986]
This study demonstrates that LdHemAC maintains an alternative pool of cAMP, which regulates antioxidant gene expression to rescue Leishmania parasites from oxidative stress.
Adenosine 3',5'-cyclic monophosphate (cAMP) is a stress sensor molecule that transduces the cellular signal when Leishmania donovani moves from insect vector to mammalian host. At this stage, the parasite membrane-bound receptor adenylate cyclase predominantly produces cAMP to cope with the oxidative assault imposed by host macrophages. However, the role of soluble adenylate cyclase of L donovani (LdHemAC) has not been investigated fully. In the present investigation, we monitored an alternative pool of cAMP, maintained by LdHemAC. The elevated cAMP effectively transmits signals by binding to Protein Kinase A (PICA) present in the cytosol and regulates antioxidant gene expression and phosphorylates several unknown PKA substrate proteins. Menadione-catalyzed production of reactive oxygen species (ROS) mimics host oxidative condition in vitro in parasites where cAMP production and PKA activity were found increased by similar to 1.54 +/- 0.35, and similar to 1.78 +/- 0.47-fold, respectively while expression of LdHemAC gene elevated by similar to 2.18 +/- 0.17-fold. The LdHemAC sense these oxidants and became activated to cyclize ATP to enhance the cAMP basal level that regulates antioxidant gene expression to rescue parasites from oxidative stress. In knockdown parasites (LdHemAC-KD), the downregulated antioxidant genes expression, namely, Sod (2.30 +/- 0.46), Pxn (2.73 +/- 0.15), Tdr (2.7 +/- 0.12), and Gss (1.57 +/- 0.15) results in decreased parasite viability while in overexpressed parasites (LdHemAC-OE), the expression was upregulated by similar to 5.7 +/- 0.35, similar to 2.57 +/- 0.56, similar to 4.7 +/- 0.36, and similar to 2.4 +/- 0.83, respectively, which possibly overcomes ROS accumulation and enhances viability. Furthermore, LdHemAC-OE higher PKA activity regulates phosphorylation of substrate proteins (-56 kDs in membrane fraction and similar to 25 kDs in the soluble fraction). It reduced significantly when treated with inhibitors like DDA, Rp-cAMP, and H-89 and increased by similar to 2.1 +/- 0.28-fold, respectively under oxidative conditions. The LdHemAC-KD was found less infective to RAW 264.7 macrophages and more prone to oxidative damage as compared to LdHemAC-OE and control parasites. Together, this study demonstrates mechanistic links among LdHemAC, cAMP, and PKA in parasite survival and invasion under host oxidative condition.
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