期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 25, 期 20, 页码 9513-9522出版社
WILEY
DOI: 10.1111/jcmm.16891
关键词
apoptosis; gastric cancer; mitochondria; piperine; ROS
资金
- Public Welfare Category of Zhejiang Science and Technology Department-Animal project [2017C37174]
- Zhejiang Medical and Health Science Technology Project [2019KY216]
Piperine (PIP), the main active ingredient in pepper, has various functions including anti-oxidation, immune regulation, anti-tumour, and promotion of drug metabolism. Studies show that PIP can inhibit proliferation of human gastric cancer cells and induce apoptosis, with mechanisms involving ROS increase, mitochondrial damage, and modulation of key apoptotic proteins. The generation of ROS by PIP promotes activation of the mitochondrial apoptotic pathway, exerting anti-tumour effects.
Piperine (PIP), the main active ingredient in pepper, belongs to the cinnamamide alkaloid. PIP has been found to have functions, including anti-oxidation, immune regulation, anti-tumour and promotion of drug metabolism. The present study was mainly designed to reveal the anti-tumour effect of PIP against gastric cancer and the relevant mechanism. In brief, the undifferentiated human gastric cancer cell HGC-27 was used, which was treated with different concentrations of PIP. As a result, PIP could inhibit proliferation and induce apoptosis of HGC-27 cells in a dose-dependent manner. The mechanism of PIP was associated with ROS increase and mitochondrial damage, simultaneously, the expression of key proteins of apoptosis was affected, including Bcl-2, Bax, Cyt-c, Caspase-9 and Caspase-3. Pre-treatment of ROS scavenger NAC HGC-27 cells could significantly reduce PIP-induced apoptosis and inhibit the activation of apoptotic signals. Consistently, PIP could induce ROS to increase and activate apoptotic signals in the animal model. Therefore, the present study showed that PIP can induce the generation of ROS, thereby promoting the activation of mitochondrial apoptotic pathway and exerting anti-tumour effects.
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