Kv2 channel-AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking

The Kv2 channels encode delayed rectifier currents that regulate membrane potential in many tissues. They also have a non-conducting function to form stable junctions between the endoplasmic reticulum and plasma membranes, creating membrane contact sites that mediate functions distinct from membrane excitability. Therefore, proteins that interact with Kv2.1 and Kv2.2 channels can alter conducting and/or non-conducting channel properties. One member of the AMIGO family of proteins is an auxiliary beta-subunit for Kv2 channels and modulates Kv2.1 electrical activity. However, the AMIGO family has two additional members of similar to 50% similarity that have not yet been characterized as Kv2 beta-subunits. In this work, we show that the surface trafficking and localization of all three AMIGOs are controlled by their assembly with both Kv2 channels. Additionally, assembly of each AMIGO with either Kv2.1 or Kv2.2 hyperpolarizes the channel activation midpoint by similar to 10 mV. However, only AMIGO2 significantly slows inactivation and deactivation, leading to a prolonged open state of Kv2 channels. The co-regulatory effects of Kv2s and AMIGOs likely fine-tune both the electrical and non-electrical properties of the cells in which they are expressed.

Automated summary

Kv2 channels encode delayed rectifier currents that regulate membrane potential and also form stable junctions with AMIGO proteins to modulate conducting and non-conducting channel properties. Among the AMIGO family, AMIGO2 significantly slows inactivation and deactivation of Kv2 channels, leading to a prolonged open state. The co-regulatory effects of Kv2s and AMIGOs likely fine-tune both the electrical and non-electrical properties of expressed cells.