miR-6718-5p and miR-4329 can be used as potential biomarkers for acute myocardial infarction

Background The prevention and prognosis of the onset or recurrence of acute myocardial infarction (AMI) is a difficult problem in contemporary research. Methods In this study, peripheral blood samples were collected from seven patients with AMI and nine healthy adults, and exosome microRNAs (miRNAs) were extracted. The miRNA differential expression profiles of serum exosomes in patients with AMI were obtained by using the next-generation sequencing technology combined with bioinformatics analysis. Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to verify the primary screening of differential exosome miRNAs to reveal the possible mechanism of their action on AMI. Results Compared with healthy individuals, 544 miRNAs were upregulated and 518 miRNAs were downregulated in AMI patients preoperatively. Among these miRNAs, we selected miR-6718 and miR-4329 for qPCR verification. The expression of miR6718 and miR-4329 in patients with myocardial infarction was significantly lower than that in normal controls.

Automated summary

This study investigated the differential expression profiles of exosome microRNAs in patients with AMI compared to healthy individuals, identifying miR-6718 and miR-4329 as significantly downregulated in AMI patients. The findings shed light on the potential mechanisms involved in AMI and provide insights for further research in this area.